Sasha Levy
@SashaFLevy
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Group Leader
CSO, BacStitch DNA, Inc.
Joined November 2013
5/ 🎙️ Curious to learn more? Join us for a webinar on September 26 at 9am Pacific. We will discuss the preprint and explore how BacStitch can transform your access to long and complex DNA constructs. Register now! 👇 https://t.co/Ry6TqjQLRW
bacstitchdna.com
Register to attend here
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4/ 🧩 The platform’s composability allows the reuse and repurposing of DNA blocks without PCR. It’s DNA programming on a whole new level—perfect for rapid design-build-test cycles.
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3/ 📊 In vivo barcoding of DNA constructs makes sequence verification with Nanopore long-read technology simple and cheap.
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2/ 🌐 We’ve demonstrated assembly of DNA constructs up to 23 kb, overcoming common challenges like high GC content and secondary structure. Perfect for tackling biosynthetic gene clusters and repeat sequences!
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1/ 🔬 The platform accelerates DNA engineering by seamlessly stitching DNA blocks together in cell arrays or pools. It’s ideal for large assembly projects.
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🚀🧬 I’m excited to announce that we just published a preprint on our DNA SCRIVENER platform, a high-throughput in vivo DNA stitching tool that simplifies large and complex DNA construct assembly. https://t.co/seVn7OTYod👇
biorxiv.org
To reduce the operational friction and scale DNA engineering, we report here an in vivo DNA assembly technology platform called SCRIVENER ( S equential C onjugation and R ecombination for I n V ivo E...
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5/ 🎙️ Curious to learn more? Join us for a webinar on September 26 at 9am Pacific. We will discuss the preprint and explore how BacStitch can transform your access to long and complex DNA constructs. Register now! 👇 https://t.co/Ry6TqjQLRW
bacstitchdna.com
Register to attend here
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0
0
4/ 🧩 The platform’s composability allows the reuse and repurposing of DNA blocks without PCR. It’s DNA programming on a whole new level—perfect for rapid design-build-test cycles.
1
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3/ 📊 In vivo barcoding of DNA constructs makes sequence verification with Nanopore long-read technology simple and cheap.
1
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2/ 🌐 We’ve demonstrated assembly of DNA constructs up to 23 kb, overcoming common challenges like high GC content and secondary structure. Perfect for tackling biosynthetic gene clusters and repeat sequences!
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1/ 🔬 The platform accelerates DNA engineering by seamlessly stitching DNA blocks together in cell arrays or pools. It’s ideal for large assembly projects.
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New paper from our lab on genome-scale analysis of interactions between genetic perturbations and natural genetic variants. Congrats on the nice work, Joe! Great collaboration with the @SashaFLevy and @LarsMSteinmetz labs. https://t.co/IptOSUIo6U
nature.com
Nature Communications - Here the authors investigate the interactions between genetic perturbations and segregating loci on a genome scale in yeast using barcoded CRISPRi. Strains’ responses...
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Lots of cool findings, including the identification and characterization of hub loci that control the phenotypic effects of many perturbations. Here's the pdf:
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It uses genomic double barcoding to assay for interactions between genetic perturbations and segregating genetic loci at a massive scale.
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New paper in Nature Communications. This is the last from an awesome collaboration with Ian Ehrenreich's lab at USC when I was at Stanford.
link.springer.com
Nature Communications - Interactions between genetic perturbations and segregating loci can cause perturbations to show different phenotypic effects across genetically distinct individuals. To...
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New paper in Nucleic Acids Research. We use in vivo barcoding to reduce the cost and hands-on time of library prep for Oxford Nanopore plasmid sequencing. https://t.co/uEGo5gaySV
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Our new startup in South San Francisco is hiring a Staff Scientist, Bacterial Engineering R&D, to help develop a technology platform to engineer DNA at scale. Reach out to me with a CV if interested.
drive.google.com
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Genetic background influences the additive, dominance, and epistatic effects of many polymorphisms, including those large effect loci that always show up in mapping studies and appear 'additive'. Congrats Takeshi Matsui, @MartinMullis2, and @SashaFLevy. https://t.co/Loeb6ypBqQ
nature.com
Nature Communications - Heritable traits can be affected by additive, dominance, and epistatic effects at genetic loci. Here, the authors use chromosomally-encoded barcodes to perform linkage...
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Tech job: https://t.co/jrOHhF3DkT Postdoc job: https://t.co/wiYofmhaNy
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