RT @loicaroyer: 🚨Big news! Five years in the making, our Zebrahub paper is now published in #Cell 🎉. We’ve built a timecourse atlas of zebr….

RT @biorxiv_bioinfo: Challenges and Progress in RNA Velocity: Comparative Analysis Across Multiple Biological Contexts .

8/8 Read the full preprint to dive deeper into our methods and results. Your feedback and questions are welcome! Many thanks for the amazing mentorship from @Merlin_Lange, @ale_agranados, @sculptorofdance and @loicaroyer #AcademicTwitter #RNAvelocity #ScienceTwitter.

7/8 The choice of RNA velocity method can significantly impact the insights gained from scRNA-seq data. We hope our benchmark helps researchers choose the best tool for their data. Many thanks to #CZBiohubSF for their support! @czbiohub #OpenScience #Bioinformatics.

6/8 Our benchmark highlights the robustness of RNA velocity methods to sequencing depth. Velocyto emerged as the most robust method. We evaluated the robustness of each method's directionality predictions by comparing the subset reads with 100% of the data.

5/8 Only one gene, Pdx1, was consistently identified as a top driver across all five RNA velocity methods for the pancreas beta lineage. This highlights the variability in driver gene predictions and the importance of multi-method approaches. #Genomics #pancreas #CellRank

4/8 We observed significant discrepancies in RNA velocity predictions among different methods. We explore the agreement (or lack thereof) between methods for the zebrafish neuromesodermal progenitor dataset. #Bioinformatics #zebrahub

3/8 Local neighborhood consistency in RNA velocity methods varies by cell type. High consistency is often seen as a positive indicator, but low consistency may indicate cellular heterogeneity.

2/8 We analyze the performance of five RNA velocity methods across three developmental contexts. RNA velocity methods vary significantly in their predictions. Understanding these differences is crucial for trajectory inference. #single-cell #devbio #zebrafish #pancreas

1/8 Excited to share our preprint on RNA velocity! 🚀🧬 In this study, we compare 5 RNA velocity methods across 3 datasets. Check out our findings! #scRNAseq #RNAvelocity 📄

RT @loicaroyer: We present #Zebrahub: a timecourse atlas of zebrafish embryonic development, combining #scRNAseq time-course data with #lig….