Would you like to do high-dimensional "omics" phenotyping but on a living sample? Time to have an ESPRESSO! ☕️. After many years, we have established a framework for spatiotemporal omics name ESPRESSO!!. Full tweetorial below! Please share and RT 1/11.

RT @UniGenova: Il paper di Melody Di Bona, laureata magistrale in #Biotecnologie e dottore di ricerca in #Fisica all'Università di Genova,….

What do you want to know?.

Unique chance to work with @AD1959 himself!! Lots and lots of cool microscopy here1 .

Check out this opportunity! Great lab and cool science!.

RT @VicidominiLab: PhD Opportunity in Advanced Microscopy. If you're passionate about building innovative instruments and thriving in a han….

This work is just a little peek into our (mine and @Giulia_Tedeschi's) future lab in Toulouse at @crctoncopole ! Stay tuned for more cool insights on organelles and their role in breast cancer!! 11/11.

This work wouldn't have been possible without awesome colleagues and collaborators such as @michelledigman, Enrico Gratton, @jenn_prescher @atwoodlab Mariana Navarro, Yunlong Jia and of course the best spheroid-maker one could possibly have, @Giulia_Tedeschi! 10/11.

We performed a 16h timelapse of triple-negative breast cancer spheroids undergoing collagen-invasion, not only characterizing the presence and properties of invading phenotypes but also finding a way to figure out whether a treatment is specifically targeting them or not! 9/11

Finally, why limiting ourselves to two dimensions, when a lot of cool systems are 3D? Of course we didn't! 8/11.

We performed a 24-hour timelapse of M1 polarization of macrophages with a gene expression reporter, finding that polarization-specific phenotypes are indeed overexpressing a polarization-specific gene! We now have a direct connection to explore with other omics techniques! 7/11

Now, the question is, can organelle phenotypes be related to phenotypes traditionally defined by gene or protein expression? 6/11.

What about time? Here's a 23-hour timelapse of keratinocytes undergoing calcium-induced differentiation! We show not only massive phenotypic changes but also notice the cells organizing in patches while differentiating, showing the importance of the spatiotemporal context! 5/11

We show that cell lines have different organelle phenotypes, but what about finer differences like stress response?. We exposed our MCF10A cells to a number of stressor showing different mechanisms depending on the stressor as well as unique, stress-dependent phenotypes! 4/11

Combining hyperspectral imaging, environment-sensitive organelle dyes, phasors, bioimage and data analysis, we created ESPRESSO (Environmental Sensor Phenotyping RElayed by Subcellular Structures and Organelles) that can track cell states over 24 hours continuously! 3/11

High-dimensional phenotyping with transcriptomics or proteomics are "snapshots" in time, how can we obtain a similar wealth of information, but time-resolved? . Lots of papers hint at organelle properties and their link to cell state, can it be something to exploit? 2/11.

Last day of experiment of our last week at @UCIrvine @uci_bme (Zot! Zot! Zot!) ! Exciting times passed here, even more exciting times ahead @crctoncopole. PS: stay tuned for a boatload of papers 😃

RT @LorenzoScipion3: 🚨Postdoc position announcement!!! Please share and RT.🚨.The Scipioni Lab is seeking a highly motivated and skilled pos….

Application Materials: CV including a list of selected publications, cover letter and contact information for at least two references. Please submit your application materials via email to Dr. Lorenzo Scipioni at scipioni.lorenzo[at]4/4.

and here: 3/4.

Examples of these technologies can be found here: 2/4.