Tim Lohman
@Lohman_Lab
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Professor at Washington University in STL uses biophysics to study mechanisms of protein-DNA interactions, helicases and SSB proteins. views are my own.
Washington University in STL
Joined December 2021
Just published in PNAS. A great collaboration between the @GalburtLab and @Lohman_Lab led by graduate student Ankita Chadda .PNAS February 22, 2022 119 (8) e2114501119;
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RT @DisavowTrump20: Jeff Bezos has forced The Washington Post - the newspaper he owns - to not endorse in a presidential race for the first….
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RT @BiophysicalSoc: Janice L. Robertson to Receive 2025 Agnes Pockels Award in Lipids and Membrane Biophysics.@WUST….
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RT @CWolberger: We are hiring! Position open for a new assistant professor in the Department of Biophysics & Biophysical Chemistry in @Hop….
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Conformational change in transitioning from the inactive UvrD1 monomer-DNA structure to the leading subunit of the activated UvrD1 dimer-DNA structure.
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The same 2B domain interface occurs in E. coli UvrD dimers suggesting that this is a general interface for SF1A helicases, including Rep and PcrA.
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These structures show a massive conformational change compared to monomeric UvrD-DNA junction structures. Dimerization occurs via the 2B domains of each subunit which activates the helicase by preventing the inhibitory 2B domain-DNA interactions in the monomer-DNA structure.
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Happy to share our collaborative work with Eric Galburt's lab led by Dr. Ankita Chadda and Dr. Binh Nguyen describing the first structures of dimeric superfamily 1A UvrD-family helicases from M. tuberculosis bound to a DNA junction.
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Hetero-dimerization of a wild type and mutant superfamily 1 DNA translocase, neither of which possess DNA helicase activity, results in an active DNA helicase by relieving the auto-inhibition within the monomeric enzyme. Hopefully this link will work.
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Hetero-dimerization of a wild type and mutant superfamily 1 DNA translocase, neither of which possess DNA helicase activity, results in an active DNA helicase by relieving the auto-inhibition within the monomeric enzyme.
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RT @theNASciences: #NASmember E. Peter Geiduschek was a pioneer of DNA structure and gene expression, and to those who knew him, a "model o….
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RT @arnegennerich: Join our team! We are seeking passionate postdocs for groundbreaking research on cytoskeletal motor proteins at @Einstei….
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In good conscience, I cannot remain on this platform due to the despicable nature of the owner. You can find me on Blue Sky (@Lohman.bsky.social).
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This also indicates that ssDNA translocation does NOT limit the rate of DNA unwinding and that the nuclease domain may affect the rate of base pair melting.
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RecB nuclease domain regulates DNA unwinding by RecBCD helicase, but not single stranded DNA translocation.
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