David Breslow lab
@DavidBreslowLab
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Cilia, centrioles, signaling & cell cycle - Yale University - Molecular, Cellular & Developmental Biology
New Haven, CT
Joined June 2017
Great work by all authors, especially first Breslow lab grad student Shane Elliott. And thanks also to excellent collaborations at Yale with labs of Angelique Bordey and Tony Koleske
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3) it is noteworthy that a GOF CRISPRa screen was able to uncover a connection to a disorder caused by somatic activating mutations; possibly CRISPRa screening may be broadly useful for studying such diseases caused by somatic mutations ...
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Some implications: 1) aberrant cilia disassembly may be a recurrent feature of FCD that could contribute to pathogenesis, 2) excess cilia disassembly may be pathologic and could be considered a non-canonical basis for ciliopathy (typically caused by impaired formation of cilia),
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As FCD is often caused by somatic GOF mutations, we wondered if aberrant cilia disassembly may occur in FCD. We found that FCD-linked mutations in SARM1 or RhoA are GOF variants that promote cilia loss. Cilia loss via SARM1 also occurs with mTORC1 hyperactivation seen in FCD...
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we defined a cilia disassembly pathway formed by GPCRs [F2R/LPAR1], SARM1 NADase, centrosomal Ca signaling, & RhoA. This pathway is necessary and sufficient for cilia disassembly. Serendipitously, we noticed overlap between pathway components and genes mutated in an FCD cohort...
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Excited to share our latest work on a new cilia disassembly pathway & a link between this pathway and the neurological disorder focal cortical dysplasia: https://t.co/39JHWrtcCL Summary: From a genome-wide CRISPRa GOF screen to identify negative regulators of ciliary signaling...
science.org
A gain-of-function screen uncovers a cilia disassembly pathway linked to genes somatically mutated in neurodevelopmental disease.
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Calling all biologists! 📢 Is your research at the cutting edge of synthetic biology, genomics or AI? Apply to join our team of world-class scholars!🔬🦠 Tenure-track Assistant Professor Anticipated Start Date: July 1, 2026 #hiring #genomics #biology
https://t.co/HHDdpRQwZH
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NIH indirect costs fund the backbone of research: maintaining labs, ensuring safety, and supporting admin work. These are essential for groundbreaking discoveries. Drastic cuts to NIH indirect rates are detrimental to academic biomedical research.
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Proud to share our work by a fantastic team, spear-headed by PhD student @Kainerox with major contributions by #xenopus expert @RosiFeistel. We developed an improved iAPEX-based #ProximityLabeling method for #massspec based subcellular #proteomics. https://t.co/0jDlrlkK7M
biorxiv.org
Ascorbate peroxidase (APEX) is a versatile labeling enzyme used for live-cell proteomics at high spatial and temporal resolution. However, toxicity of its substrate hydrogen peroxide and background...
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MCDB at Yale is seeking to fill a tenured Associate/Full Professor position. Candidates must have a Ph.D or equivalent degree w/proven record of outstanding research. Review of applications begin on Nov 1. Apply at
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Delighted to share my latest #devbiol story @NautilusMag. This one’s about James Bond, cilia, and the wondrously complex interplay of embryos and people.
nautil.us
A wild tale of how scientists unraveled embryology's most fascinating mystery.
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WE ARE HIRING! MCDB at Yale is seeking to fill a tenured Associate/Full Professor position. Candidates with interest in investigating outstanding questions in biology at the organismal, cellular or molecular level are encourage to apply. Apply at
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@CurrentBiology @Armanduuum @CentrioleLab Special thanks and congrats to Anil @gangaanilkumar and Lauren Sweeney for leading this project! Also check out talk tomorrow on this work in @DGZ_info cilia / centrosome workshop (looking forward to joining great lineup of presentations!).
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Our work on PPP2R3C-MAP3K1 at centrosomes is now out in @CurrentBiology! https://t.co/D1qmbSo7JY. Now with more functional analyses (lethality of PPP2R3C KO or FOP KO is suppressed by MAP3K1 KO) and more beautiful U-ExM expansion microscopy by @Armanduuum and @CentrioleLab!
Excited to share our preprint on a new centriolar kinase-phosphatase module, with fantastic U-ExM microscopy by @CentrioleLab and @Armanduuum! https://t.co/Ti4hKdzyQ1 Here we studied Ppp2r3c, a PP2A phosphatase regulatory subunit and hit from our prior cilia CRISPR screen…
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Looking forward to participating in this exciting session!
Our #Cilia & #Centrosome Workgroup led by @DagmarWachten and @MickLab will kick-off our 3rd round of #DGZ Focus Workshop: Sept 24, starting at noon, zoom, with an exciting speaker list ⬇️ join us!
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Congratulation to Shane Elliott - the first grad student of the Breslow lab - on his PhD graduation and receiving the Yale MCDB Department Spangler thesis award!
Congratulations to the2024 Spangler Award Recipients! To see more about the award recipients, visit https://t.co/ybC9pBt91X
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Please join us in congratulating Ilana Richman, MD, recipient of this year's Best Published Research Paper of the Year Award! Congratulations, Ilana! #SGIM24 @IlanaRichman
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And he is holding what might be the best poster prize ever: a 3D-printed cilia trophy crafted by @ReiterLab. Thanks to Jeremy, @Mill_lab , and Peter Jackson for organizing a fantastic meeting!
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Had a great time at the always excellent FASEB Cilia conference - and especially excited that Breslow lab postdoc Jingbo Sun won the first place poster award!
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This was all in human cells, but evidence points to a functionally analogous PP2A-PPP2R3C-FOP-CEP350 controlling MT org in plants, which don’t even have centrosomes! Thus, PPP forms an evolutionarily ancient phosphatase module conserved across divergent modes of MT organization.
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