CRISPRGuru
@CRISPRGuru
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Genome Editor. Co-developer of Easi-CRISPR; https://t.co/ZfCN519CzC. World now needs peace and solidarity, not war. Opinions mine.
Omaha, NE
Joined March 2011
I received these award-plaques recently, in a ceremony at my institute, for innovating a better CRISPR method called Easi-CRISPR. See link for a short story of how it came to be.
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Generate videos in just a few seconds. Try Grok Imagine, free for a limited time.
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I am excited to be speaking about novel CRISPR genome engineering methods at Discovery Europe 2024 conference, May 22-23, Basel, Switzerland.
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Sunday Brunch: .Jowar Roti (ಜೋಳದ ರೊಟ್ಟಿ) and Stuffed brinjal/Badanekayi Ennegayi (ಬದನೆಕಾಯಿ ಎಣ್ಣೆಗಾಯಿ)
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The Sky is blue and the Sun is bright now in Nebraska, before the eclipse. It will be partial here though!
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Looking forward to speaking about "Emerging Technologies in Genome Editing" in two weeks at the ABRF 2024 meeting.
We had such great leadership and insights from @CRISPRGuru, who was an invited panelist to discuss genome editing! #UNMCResearch #ABRF2023
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Sunday morning breakfast:.Rava Idli (ರವೆ ಇಡ್ಲಿ) and Coconut-Peanut Chutney (ತೆಂಗಿನಕಾಯಿ-ಶೇಂಗಾ ಚಟ್ನಿ), all made from scratch.
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This new (simplified) method is a new addition to other short- and long- range sequencing based approaches, like CRISPR-KRISPR and CRISPR-LRS .
genomebiology.biomedcentral.com
CRISPR tools can generate knockout and knock-in animal models easily, but the models can contain off-target genomic lesions or random insertions of donor DNAs. Simpler methods to identify off-target...
A simplified method to find out if DNA inserted correctly in your CRISPR mouse .3 Steps: Isolate genomic DNA, enrich target region via Cas9 (cut & ligate adapters) & long range sequence. Shown here for AAV CRISPR KI mice: can be used for any CRISPRed mice.
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A simplified method to find out if DNA inserted correctly in your CRISPR mouse .3 Steps: Isolate genomic DNA, enrich target region via Cas9 (cut & ligate adapters) & long range sequence. Shown here for AAV CRISPR KI mice: can be used for any CRISPRed mice.
biorxiv.org
Over the last decade CRISPR gene editing has been successfully used to streamline the generation of animal models for biomedical purposes. However, one limitation to its use is the potential occurr...
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CRISPR guide RNAs & DNA primers are widely used reagents in biomedical research. Are these reagents always pure, without any contaminants? . Probably not! . Here is our analyses of reagents procured from several major suppliers in Europe, Asia and USA.
academic.oup.com
Abstract. Custom oligonucleotides (oligos) are widely used reagents in biomedical research. Some common applications of oligos include polymerase chain rea
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A new wave of CRISPR-delivery technologies? .See link for info.
biorxiv.org
Therapeutic application of CRISPR-Cas9 is limited by the available approaches for delivering genome editing tools to specific cell types. Enveloped delivery vehicles (EDVs), such as virus-like...
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If scientists get ‘curious’ and follow-up why some of their experiments showed weird results—instead of thinking their experiments did not work—is when new unexpected (but potentially breakthrough) discoveries are made. You will see such an example in this tweetorial!.
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This is an 'exceptional technological advance' in turning on/off of gene expression at mRNA levels. Very impressive! .
nature.com
Nature Biotechnology - Mammalian gene expression is controlled by an RNA switch system.
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This was one of the most successful workshops on CRISPR Genome Editing in Mice: GONAD and microinjection. More details in the link below.
Three day hands on workshop on CRISPR mouse genome engineering technologies (GONAD and microinjection) began today at the Van Andel Institute, Grand Rapids, Michigan.
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RT @nevillesanjana: TWEETORIAL time! In new work, we identify required transcription factors for neural differentiation using a TFome-wide….
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RT @nevillesanjana: There is much more in the paper, which is available here (Open Access):. This was one of the fi….
nature.com
Nature Communications - Using integrative multi-omics and CRISPR knock-out of all ~1,900 transcription factors, the authors identify essential transcription factors required for Neurogenin-driven...
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Three day hands on workshop on CRISPR mouse genome engineering technologies (GONAD and microinjection) began today at the Van Andel Institute, Grand Rapids, Michigan.
GONAD (Genome editing via Oviductal Nucleic Acids Delivery) is a powerful technology developed by @Masato_Ohtsuka. Many students ask us for a training opportunity. Here is a workshop at @VAInstitute .. Limited spots!. Secure your's before they are gone!.
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It was fun meeting and interacting with nearly 60 participants at the workshop on CRISPR technologies, offered yesterday at AIIMS, New Delhi.
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Links to the conference and the workshop websites.
Looking forward to offering this workshop on CRISPR genome editing, sponsored by ISTT and ISTR. Venue: All India Institute of Medical Sciences, New Delhi, India. Date: October 15, 2023.
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