R. Graham Cooks
@AstonlabsPurdue
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West Lafayette, IN
Joined March 2018
We’ve had a busy year (perhaps too busy to post consistently!), so here goes a recap:
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As well as a tutorial on quantitation via direct MS analysis! @ACSPublications
pubs.acs.org
This tutorial is intended as an educational resource covering the application of mass spectrometry (MS) to quantitative analysis. MS is a widely used instrumental technique in both academic and...
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Lastly, and fresh out of the oven, we have reviewed the role of ambient ionization MS in brain cancer diagnosis @JMSACL
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And facilitating directed evolution efforts, for instance towards small-molecule phosphorylation (in collaboration with Prof. Lewis @IUBChemistry) @angew_chem
onlinelibrary.wiley.com
PsiK from Psilocybe cubensis phosphorylates a diverse set of substituted phenols and benzenediols beyond its native substrate. High-throughput screening of active site mutant libraries using DESI-MS...
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The rapid characterization and countermeasure development against chemical warfare agents (in collaboration with @Livermore_Lab) @PNASNews
pnas.org
The recent alleged use of A-series chemical warfare agents (CWAs) highlights the urgent need to better understand their inhibition of cholinesteras...
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As well as its application for direct and label-free biological assays which can be used to characterize new biological targets, such as Drosophilla Achy as a redox sensor (in collaboration with Prof. Weake @PurdueBiochem) @PNASNews
pnas.org
One-carbon metabolism influences gene expression by providing methyl units for DNA, RNA, and histone methylation. Robust methylation requires rapid...
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Going back to high-throughput experimentation, lately we have also been focused on showing the wide range of applications of the HT-DESI-MS technology. One example is the synthesis and characterization of PFAS analogs @EnvSciTech
pubs.acs.org
Per- and polyfluoroalkyl substances (PFAS) are a global challenge due to their exceptional thermal and chemical durability which leads to environmental persistence, bioaccumulation, and toxicity....
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And implementing 2D-MS/MS for de novo sequencing of peptide mixtures via network analysis! @ChemicalScience
pubs.rsc.org
Two-dimensional tandem mass spectrometry (2D MS/MS) provides in-depth biopolymer structural information previously not directly accessible with traditional one-dimensional MS/MS workflows, and in...
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We are also very interested in chemical analysis! For instance making improvements to paper spray:
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And, as we are so deeply interested in these microdroplet phenomena, we wrote a perspective summarizing our thoughts! @ChemicalScience
pubs.rsc.org
Over the past decade the use of electrospray ionization mass spectrometry (ESI-MS) has been extended from the domain of chemical analysis to that of chemical synthesis. This development was spurred...
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We have also studied the chemoselectivity of some of these microdroplet reactions: @ChemicalScience
pubs.rsc.org
Dehydration reactions are typically favored in microdroplets due to the relatively dry environment at the air–water interface. However, we demonstrate that cyclodesulfurization may outcompete...
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Thanks to @PurdueChemistry for the highlight to this work!
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And the fixation of nitrogen via in situ lithium nanoparticle generation! @ChemicalScience
pubs.rsc.org
Multiphase reduction of ambient dinitrogen (N2) was observed during the deposition of charged or uncharged aqueous microdroplets containing a lithium salt pre-catalyst onto conducting or inert...
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As well as the green synthesis of pyrazoles (in collaboration with Prof. Pradeep from @iitmadras) @ACSSustainable
pubs.acs.org
Microdroplets containing water in the presence of an electric field can generate superacids/superbases at the gas–liquid interface, and this unique property drives organic reactions within millisec...
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We have also explored novel applications of microdroplet chemistry, for instance towards the accelerated synthesis of new heterocycles. @ChemicalScience
pubs.rsc.org
Click chemistry is important for its simplicity and versatility, however, condensation-based click reactions are limited by the general requirement of high temperatures and catalysts. Here, we report...
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As well as their role, together with serine octamer clusters, in the origin of homochirality. @JPhysChem
pubs.acs.org
The chemistry underlying biological homochirality remains challenging. We propose that noncovalent clusters may have served as the means by which an enantiomeric excess of one amino acid could direct...
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We’ve also continued exploring the implications of accelerated microdroplet reactions in the origin of life, for instance on the abiotic synthesis of saccharides. @ChemicalScience
pubs.rsc.org
Understanding the chemical reactions that led to the origin of life is a fundamental challenge of chemistry. The formose reaction, an abiotic pathway to monosaccharides, provides a mechanism of sugar...
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This making-testing process has been certainly sped up by using DESI to collect microgram amounts of new drug analogs in an automated array-to-array fashion! @ChemicalScience
pubs.rsc.org
Automation of chemical synthesis and high-throughput (HT) screening are important for speeding up drug discovery. Here, we describe an automated HT picomole scale synthesis system which uses desorp...
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We have continued our work on implementing high-throughput DESI-MS as a rapid strategy for screening reactions and testing new molecules! @ChemPlusChem
chemistry-europe.onlinelibrary.wiley.com
Accelerated drug derivatization in microdroplets combined with high-throughput desorption electrospray ionization mass spectrometry provides rapid reaction screening and label-free bioassay results,...
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