Sebastian S. Cocioba🪄🌷
@ATinyGreenCell
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Amateur 🌿 Biologist • Aspiring 🌺 Designer Researcher at Binomica Labs • Degreeless Heathen • Petunia Breeder • Open Lab Notebook: https://t.co/umYTLluuuw
Huntington, NY
Joined October 2009
Excited to announce some new plasmid offerings in my shop at https://t.co/ScJPW2CH0V! Here's a thread of the current catalog. All proceeds directly support my lab and my continued effort to build useful open-source tools. Feel free to get in touch if you want to know more 💚 1/7
atinygreencell.com
Designer of fine, hand-crafted, bespoke, and fully open source biotechnology tools for educators and researchers. Plasmids, lab equipment, cell lines, and seeds. An ever-growing collection of high...
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Friday Flower 004: Hibiscus trionum 🌺✨ Hibiscus trionum displays a dark bullseye in the middle that acts as a landing pad for pollinators 🎯 The bullseye is made by developmental boundary delineating distinct cell shapes and pigments.
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Some good news: the NIH has now reinstated my R35 since the termination was found unconstitutional (still waiting on NSF), though the government continues to withhold the contractually obligated funds
Yesterday, the NIH R35 “Outstanding Investigator” grant to fund scientists in my lab studying antibiotic resistance was terminated for reasons not related to the content of the science, or any actions taken by me or members of my lab
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How rough of a childhood did folks like Ben have to think so cynically of humanity? Genuinely sad to see this sort of attention-seeking, esp when its baked into a marketing strat. Obv this is a symptom of an increasingly apathetic populace. We can do better. We must do better.
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This is an amazing story of scientific progress When it was first described, life expectancy in cystic fibrosis was about 7 months Today, since discovery of the causative gene (CFTR) and therapies that modulate it (like Trikafta), life expectancy is almost normal
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Mutation in maize chloroplastic polypeptide chain release factor (ZmcpRF1) affects chloroplast development and leaf color. Chen, H., Dong, H., Shan, X. et al. Planta 262, 63 (2025). ➡️ https://t.co/QbKj1QMs0Z
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Leaving this here without explicit context, because everything happening in the world today is enough context. https://t.co/75eVmbHNaA
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I'm getting better at petal infiltration! Five different experiments on one bloom (with 3 repeats across different blooms).
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E. coli transcription factors regulate promoter activity by a universal, homeostatic mechanism https://t.co/SUI5Xkq1Pc
science.org
Transcription factors (TFs) may activate or repress gene expression through an interplay of different mechanisms, including RNA polymerase (RNAP) recruitment, exclusion, and initiation. However,...
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No match for zymo shield tho! It denatured it so fast even through the bacterial cell. 1mL spindown of two fluorescent protein cell lines. Right pair is FuGFP. Left tube of each pair is water neg control. Resuspended in 500uL of either water or Zymo DNA Shield. Lost color in secs
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Amazing how resilient a superfolder gfp is to harsh conditions. FuGFP on far right. Big fan of this one. Glows even when in alkaline lysis buffer!
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I am a fluorescent protein enjoyer and avid collector.
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Kimi Ishizaki, a professor of Kobe University told me that axenically cultured Marchantia polymorpha is edible in raw! Today, our students compared the taste of three species of Marchantia: M. polymorpha, M. pinnata, and M. quadrata.
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Yay, got a few agrobacterium colonies for my RUBY + cas9 plasmid! Gonna work on a nice hello world crispr experiment where you transform a petunia with this construct and then do a secondary transform with guide RNA targetting the RUBY. Stable line expressing cas9 and visual KO.
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Golden Braid my beloved
GoldenBraid2.0 E. coli: a comprehensive and characterized toolkit for enterics "We provide a complete lab manual and toolkit with 478 parts for gene expression and protein tagging." https://t.co/Aol9bbitoe
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Welcome to bioengineering
How do you program an unknown CPU? The original specs are gone; no compilers exist, and the ISA is completely unrecognized. It happens more often than you think, behind very closed doors. It's almost always military hardware.
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Your ship lands softly on a bed of grass 20m from a research station that went dark 5 days ago. Your clients asked you to investigate the outage and report back any anomalies. The ship airlock depressurizes and you walk onto the strange Moon's surface. What do you do?
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🌱 ¿Quieres realizar tu tesis doctoral desarrollando un atlas del transcriptoma single-cell y la caracterización funcional de reguladores del desarrollo y maduración del fruto de fresa? Ofrecemos un contrato de la convocatoria FPI asociado al proyecto PID2024-155863OB-I00
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