RT @VirusesImmunity: New preprint led by @JennyLund15: analysis of 232 participants shows that during HSV-2 shedding, T cells and dendritic….

RT @HelenBranswell: Helen Chu is one of the ousted #ACIP members. She spoke to me today about her grave concerns for future vaccination pol….

RT @dbacsik: 6/ Read more in our manuscript from the @GreningerLab at @UWVirology: Many people contributed to this….

RT @dbacsik: 1/ How has H5N1 affected this year’s flu season in Seattle? Despite ongoing outbreaks in birds & cattle nationally, we detecte….

RT @UWVirology: Virology now offers the H5 influenza test - !. @fredhutch @uwmedicine and @HutchIDScience @uwlabmed….

Grateful to my amazing colleagues and coauthors for contributing to this work. And our collaborators across the pond who generously shared early versions of the ARTIC primers. /end.

tl;dr Assay shows robust performance across a range of viral loads and clinical specimens. Its sensitivity makes it suitable for use in clinical trials of antivirals and vaccines allowing early detection of treatment emergent/resistance mutation. 4/

We determined sensitivity, specificity, repeatability and reproducibility; and comparison with Sanger, shotgun, and capture-based sequencing to check for accurate recovery of the F gene. We also used contrived samples to determine the LOD of minor variants in allelic mixtures. 3/

We modified 2 ARTIC primers based on initial tests w samples from WA/USA, but changes are likely improve performance in global isolates based on current variation at those sites. We were able to recover high quality genomes from samples with wide range of Cts (95% LOD Ct 30) 2/

Happy to share our latest preprint describing clinical assay validation for RSV whole genome sequencing using a modified version of the ARTIC RSV panel <thread> 1/.

Excited to be involved in this study led by @HelenChuMD as part of the @CDCgov's CHARM network tracking the spread of respiratory viruses across the US.

RT @HerpesAdvocate: News from earlier this Fall from @AssemblyBio. Thanks to @LuisSchang for this write up!.

Thanks to @HelenChuMD for inviting me to contribute to this project, and to collaborators on and off Twitter @ericchowmd @pdh_dna @lea_starita @trvrb @JShendure @BrotmanBaty /n.@uwmnewsroom.

Prior to this, there were only ~20 CVA21 genomes in Genbank, showing the value of these studies in characterizing the circulating genetic diversity of understudied respiratory pathogens which participants said affected their daily activities. 5/

We @UWVirology @GreningerLab performed sequencing using a capture-based approach and identified EV-D68, CVA21, 4, and 6. Surprising (to me) was that there was enough genetic material in environmental swabs to recover full genomes. 4/.

The goal was to characterize the epidemiology of non-rhinovirus enteroviruses in these congregate settings. We've previously done this for HPIV, RSV, seasonal human coronavirus, and rhinovirus. Here's a nice thread on one of many led by @ericchowmd: 3/.

Seattle Flu Study was a large collaborative effort aimed at understanding the transmission and prevalence of respiratory pathogens for pandemic preparedness. Samples in the current paper were collected from homeless shelters across the Seattle area. 2/.

Our paper on clinical and genomic epidemiology of EV-D68 and CVA21 in congregate settings is now online in @EIDjournal. Led by @SarahNicoleCox from @HelenChuMD's lab this work used samples and data from the Seattle Flu Study . @seattleflu_sfa 1/.

RT @fredhutch: "We're the National Institutes of Health, not the National Institutes of Not Dying," said Dr. Keith Jerome of Fred Hutch, wh….

Celebrity sighting in London. Very cool, @baym