Ramil R. NOCHE, Ph.D.
@docnitephd
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🇵🇭🇨🇦 Zebrafish and CRISPR-Cas expert, Life science leader, Macrophage and microglia enthusiast, polyglot
Toronto, Ontario
Joined January 2009
I’m thrilled to share that our paper “Machine-guided design of cell-type-targeting cis-regulatory elements” was published online in @Nature. https://t.co/NJiCihH745
#genomics #MachineLearning #SyntheticBiology #bioengineering 1/9
nature.com
Nature - A generalizable framework to prospectively engineer cis-regulatory elements from massively parallel reporter assay models can be used to write fit-for-purpose regulatory code.
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Excited to share our sequence design work published in @Nature. I had the privilege of co-leading this effort alongside my friend and colleague @SagerGosai under the supervision of @r_tewhey, @ReillyLikesIt, and @PardisSabeti
https://t.co/mJSvyaMUHT 1/7
nature.com
Nature - A generalizable framework to prospectively engineer cis-regulatory elements from massively parallel reporter assay models can be used to write fit-for-purpose regulatory code.
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We’re very excited to share the first preprint from my lab along with @h4rrymcnamara, @BillZJia, @AdamEzraCohen, and Alex Schier. We describe a set of methods for exerting spatial control over Nodal signaling in zebrafish development. https://t.co/4Opufofwop
biorxiv.org
A crucial step in early embryogenesis is the establishment of spatial patterns of signaling activity. Tools to perturb morphogen signals with high resolution in space and time can help reveal how...
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Our first preprint as a lab is out! This one is short and sweet: We thought there had to be a faster/higher throughput way of making transgenic fish, so we set out to test if lipofection works, and it does!
Lipid-Based Transfection of ZebrafishEmbryos: A Robust Protocol for NucleicAcid Delivery in Zebrafish https://t.co/mMAhbAQUXF
#bioRxiv
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2 body problem: now looking for career opportunities and will be returning soon to the Greater Toronto 🇨🇦 area to join my wife and son. Zebrafish? CRISPR-Cas? Molecular Biology? Genetics? Imaging? Project Management? All leads will be appreciated!
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Tissue and Cell type Design of Synthetic Enhancers. Two papers in @Nature by the labs of @steinaerts and @AlexanderStark8 make it possible to generate enhancers a la carte. The papers: 👇 https://t.co/oB2umkiW2K
https://t.co/ASdGcRezfC
nature.com
Nature - Deep learning models were used to design synthetic cell-type-specific enhancers that work in fruit fly brains and human cell lines, an approach that also provides insights into these gene...
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Moving the lab to Paris in 2024, the following frozen (sperms) lines will soon be thrown away. Black, blue and red are different #InHoxWeTrust alleles. Anyone interested can contact Benedicte.Mascrez@unige.ch #NoFlowersByRequest 🙏RT https://t.co/8ZTSO4CUfH
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I’m thrilled to share the results of a phenomenal collaboration with Lasse Dissing-Olesen in the @stevens1lab. If you’re hungry for microglial biology and looking for a new set of utensils with which to dig in, please read on. https://t.co/DcCb0rtEfN 1/n
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"PLCγ2 Variants Toggle Microglial Plaque Compactors." Thanks to @alzforum for the excellent summary of our @ImmunityCP paper! We appreciate the valuable comments from @LukensJohnR @HiltunenLab and thanks again to the team. I also hope that I have the P522R variant :)
Genetic variants of PLCγ2 may sway AD risk by dialing microglial management of Aβ plaques up or down. @IUMedSchool
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Excited to share our work in @ScienceMagazine - Kupffer cell-like syncytia replenish macrophage function to capture bacteria in a fibrotic liver. Huge congrats to lead authors @moritzpeiseler and @brunaraujodavid on their amazing work!! https://t.co/GtgMic8l6C
science.org
Fibrosis-associated loss of Kupffer cells is compensated by bacteria-filtering monocyte syncytia in the liver.
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PUFFFIN: A novel, ultra-bright, customisable, singleplasmid system for labelling cell neighbourhoods https://t.co/dAA1E3v2BK
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Our latest preprint is out! In a fun collaboration with @DrAnneCarpenter, we conducted a systematic high-content screen to understand the role of protein mislocalization in diverse human disorders. 1/8 https://t.co/Oebh0OaZHw
biorxiv.org
Widespread sequencing has yielded thousands of missense variants predicted or confirmed as disease-causing. This creates a new bottleneck: determining the functional impact of each variant – largely...
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Sharing extraordinary work of @martincarbotano with @MLapoix @ciliaJia_jxy @wyartlab who identified the MLR in larval zebrafish, how MLR neurons encode vigor, and project onto reticulospinal neurons throughout the brainstem to elicit forward locomotion https://t.co/Its251nb7Q
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People who fall for false information tend to be overconfident in how they think—not just what they think. Data: those who overestimate their math & reasoning skills are more susceptible to conspiracy theories. Critical thinking requires a healthy diet of intellectual humility.
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A comparative analysis of microglial inducible Cre lines
cell.com
Microglia are key regulators of neural circuits and neuroinflammation. Tools to specifically manipulate their function in vivo are critical. Faust et al. show that recombination efficiency and...
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Exciting news!!😀💥 Our work with @FeijooCarmen and @SMBurgessLab is out in @NatureComms 🎉 We investigated ontogenetic hematopoietic diversity of neutrophils in zebrafish 🦓🐟 What did we find? 🧵👇 #NewPublication #zebrafish #hematopoiesis
https://t.co/809J5aODV8
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America's antivaxx crisis: a quarter believe MMR shot causes autism; a third think Covid vaccines kill. It’s extraordinary, despite my battling antivaccine activism daily for 2 decades. The disinformation machine is so powerful and now backed politically
dailymail.co.uk
One-quarter of American adults said they believe the measles, mumps and rubella (MMR) vaccine causes autism - a widely studied and discredited claim that emerged in the 1990s.
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We @AcadSinica are recruiting for junior PIs!! 😃😃😃
OPEN FACULTY POSITION https://t.co/Vch0fmgdAM. Please join us! We have stable intramural funding until you RETIRE! For those working and studying at IMB, nothing is "impossible", our research is only limited by our imagination, and pursuing excellence is our only mission!
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Ang paniniwala ni Ninoy Aquino: lahat tayo ay may kakayanan na patuloy na mahalin, ipaglaban, at itaguyod ang mahal nating Pilipinas. Tanging sa pagkakaisa nating lahat nakasalalay ang pagtamasa ng tunay na kalayaan. #SalamatNinoy #RememberingNinoy #HindiKaNagiisa
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😌Si l’agenda de nos #enseignements ne vient pas à l’auditeur 😌L’auditeur vient à notre #agenda 👉 https://t.co/gwokQowBwB
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