My first work in Xiaowei's lab out as a preprint! I'm so honored to work with Shiwei and Pu @sclerei on revealing cell-type specific chromatin organizations in the motor cortex and contribute to the project by studying the effect of Mecp2 deletion. https://t.co/VMXrKFv8HB

Zhuang lab reunion at 4DN Annual Meeting

(7/7) Finally, we applied our technology to Mecp2+/- mice. We found that depletion of MeCP2 altered nuclear radial positioning and local A/B compartmentalization of chromatin loci in a radial-position-dependent manner in neurons, similar to its effect on gene transcription.

(6/7) Interestingly, we observed a correlation between nuclear radial positioning and the amount of bound MeCP2. Moreover, MeCP2 regulates gene expression in a radial-position-dependent manner and such effect is the strongest in excitatory neurons.

(5/7) Spatial positioning of chromatin loci along the radial axis of the nucleus also showed strong cell-type dependence. For example, active and inactive chromatin in neurons adopted a more intermixed radial positioning in the nucleus.

(4/7) Across different cell types, transcriptional activity of genes, as well as enhancer activities, were correlated with the local spatial density of compartment-A chromatin loci.

(3/7) We observed cell-type-dependent changes in higher-order chromatin structures that were correlated with transcriptional activity changes between cell types. In non-neuronal cells, chromosomes preferentially formed large domain structures that resemble the megadomains in Xi.

(2/7) We showed that different cell types had substantially different physical sizes of the cell nucleus and chromosome territories, both of which were strongly correlated with the total transcriptional activity of the cell.

(1/7) We applied integrated RNA- and DNA-MERFISH to the primary motor cortex of mice. We used RNA-MERFISH to profile the transcription of 46,340 cells and identified 21 cell types. We then determined the 3D genome organization for each identified cell type.

Thanks Pu @sclerei and Shiwei for teaching me MERFISH. And I'm also happy to make my own contribution to this project as well.

Charming video of Matt Meselson and Frank Stahl speaking about their 1958 experiment, which truly is the most beautiful experiment, and the beautiful friendship they have, and how playful and free science seemed to be back then https://t.co/WjdObOBLMg

Thrilled to finally share my PhD work, out today as a preprint! 🎉 Q: How do the brain’s dopamine and serotonin systems work together to drive learning? 🧠 Short answer, 🧵 below Long answer: 1/9

A Hypothalamic Circuit Underlying the Dynamic Control of Social Homeostasis https://t.co/yjGuunicNb #biorxiv_neursci

Work by @jhowardtrotter, @WangCosmos, @SudhofThomas & colleagues shows that a combinatorial code of neurexin-3 alternative splicing controls inhibitory synapses via a trans-synaptic dystroglycan signaling loop.

Excited to share our preprint from the Ginty lab @harvardmed and Sharma lab @Columbia. Big shoot-out to the co-authors (@MIskols and others whose twitter not known to me). We hope the DRG neuron genetic toolkit can be a useful resource for the field.

Thrilled to share our latest work from @JoeEcker lab, using cutting-edge snmC-seq3 and snm3C-seq technologies to generate 301,626 methylomes and 176,003 mC+3C multiome profiles from 117 dissected regions throughout the adult mouse brain. (1/n) https://t.co/APlIJSW7VP

The adhesion GPCRs CELSR1-3 and LPHN3 engage G proteins via distinct activation mechanisms https://t.co/LiRtxyxGfI #bioRxiv

A molecularly defined and spatially resolved cell atlas of the whole mouse brain https://t.co/dj6BW5Sncm #bioRxiv

Congratulations to Professor @CarolynBertozzi, winner of the 2022 @NobelPrize in Chemistry for her role in the development of bioorthogonal chemistry. https://t.co/xn7yg9h64O

How does the brain differentially encode signals from the visceral organs to generate our internal senses? My postdoc paper @Nature reports the coding of organs by Ca imaging and reveals a visceral homunculus in the brainstem that depends on inhibition.