John Coukos
@JohnCoukos
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MD/PhD student, UChicago | Chemistry PhD '21, Moellering Lab
Joined June 2013
ACS did a brief "Introducing Our Authors" feature for my recent publication from our work in the @rmoellering14 lab:
pubs.acs.org
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Kudos to Wendy Luo (MS4) and team (@smae_aokay @JohnCoukos @marthpodi, Tatjana Antic, and Mike Kwon) on their publication on organ-confined prostate cancer with negative margins https://t.co/1AKxMIDMzP
pubmed.ncbi.nlm.nih.gov
In this study, organ-confined PCa with negative margins in an entirely embedded RP carried no mortality risk. In particular, the patients with GG 1-2 disease may have benefited from less rigorous...
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Today in @natchembio we share a new strategy to design fully synthetic “artificial” bZIP family transcription factors that directly block DNA binding of HIF1a and XBP1 to inhibit hypoxia-dependent oncogenic signaling in cells and in vivo. 1/n https://t.co/1xIyfjbw0O
nature.com
Nature Chemical Biology - Qiao and Nguyen et al. describe a strategy to block hypoxia-dependent gene expression in cell and mouse models of breast cancer through dual targeting of X-box-binding...
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Cool covalent photoacoutstic imaging probes from the Chang lab @emory. #proudofalumni
onlinelibrary.wiley.com
We developed an NCEH1-targeted covalent photoacoustic imaging probe activated under hypoxia. This probe potently and selectively labels NCEH1, an aggressive tumor associated serine hydrolase, in...
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Excited to share our @nchembio publication! Our proximity-triggered protein trans-splicing technology enables precise, time-resolved activation of diverse proteins by manipulating control elements (1/5) https://t.co/UHgdZhxVjU
nature.com
Nature Chemical Biology - Time-resolved synthesis of target proteins via proximity-triggered protein trans-splicing has now been shown to enable the activation of a diverse set of proteins upon the...
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In the latest issue! A glycolytic metabolite bypasses “two-hit” tumor suppression by BRCA2
cell.com
Cells carrying a monoallelic germline BRCA2 mutation can bypass Knudson’s “two-hit” requirement to initiate tumorigenesis when the glycolytic metabolite methylglyoxal transiently disables BRCA2...
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Key genes and convergent pathogenic mechanisms in Parkinson disease — a Review by Robert Coukos & Dimitri Krainc https://t.co/KV4JqoC7tA
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A glycolytic metabolite bypasses “two-hit” tumor suppression by BRCA2 Very exciting finding from Ashok Venkitaraman's lab!
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Delighted to share that I’ve been promoted to full Professor @UChicago @UChiChemistry! More than anything this is a reflection of the talent and dedication of my students & postdocs, past and present, alongside support from many mentors, collaborators and the chembio community!
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Two 2023 studies point toward the #parkinson associated protein PARK7 / DJ-1 as being a glyoxalase, not a deglycase, by acting on the precursor metabolite methylglyoxal: https://t.co/cD0qVr4rtJ
https://t.co/uuYXPXFBTf
#PTM
#glycation
#AGEs
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In a major milestone for science, for medicine, and for patients, the world’s first CRISPR drug has been approved in the UK. “A new treatment for sickle-cell disease and transfusion-dependent β-thalassemia has been authorised by the Medicines and Healthcare products Regulatory
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Hello!! I have multiple positions open at @UCCancerCenter and @UChicagoBSD Metabolomics Platform. We have multiple brand new high-resolution #orbitrap #LCMS systems and very exciting #metabolomics projects. This will be a great opportunity to acquire expertise in #LCMS.
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The Moellering Lab has published research in @BiochemistryACS that provides "definitive evidence that recombinant DJ-1 produced in human cells prevents the stable glycation of other proteins through the conversion of methylglyoxal" #Parkinsons Read -
pubs.acs.org
The protein PARK7 (also known as DJ-1) has been implicated in several diseases, with the most notable being Parkinson’s disease. While several molecular and cellular roles have been ascribed to DJ-1,...
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New research from @JohnCoukos @rmoellering14 & @UChiChemistry colleagues reports the stereospecific conversion of methylglyoxal to l-lactate by #Parkinsons-associated DJ-1 in solution with negligible or no contribution of direct protein deglycation https://t.co/masNFvqewH
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Our preprint is out! We hacked the @nanopore sequencer to read amino acids and PTMs along protein strands. This opens up the possibility for barcode sequencing at the protein level for highly multiplexed assays, PTM monitoring, and protein identification! https://t.co/hVdNFa7ti4
biorxiv.org
The ability to sequence single protein molecules in their native, full-length form would enable a more comprehensive understanding of proteomic diversity. Current technologies, however, are limited...
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Stevan Micic is a 57kg WORLD CHAMPION! 🇷🇸 He upended an Olympic Champ and TWO World Champs en route to winning Serbia’s FIRST WORLD GOLD MEDAL EVER in front of the home crowd… Incredible! He sure earned it. So happy to see him seize a great opportunity, he is a national hero
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Excited to share our group's most recent work out today in @CellReports: In this paper, we discover ERK1/2 are regulated by dynamic lipidation - illuminating a new layer of control for these critical cellular kinases https://t.co/O98Vz0y2qp 1/n
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Anyone that has seen our work knows we have a different view on PTMs! Here, @MarissaNT96 and I opine that glycation is actually crucial for normal cell health and homeostasis and not simply a marker of sugar overload. Thanks to @nchembio and reviewers!
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He went 166-0 in high school. He was the top recruit for the most successful NCAA program. He was also addicted to opioids. Chance Marsteller went to jail and rehab (twice) and almost lost his wife and kids. Then he beat the best wrestler in U.S. history.
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Check out our latest work uncovering another connection between glycolysis and the KEAP1-NRF2 pathway. A novel PTM of a KEAP1 cysteine by a reactive glycolytic metabolite. Thanks to coauthors: @LeuAlaArgAla @KaylaENutsch @RosaCarolinae @LabWiseman
https://t.co/rO45tyZRim
pnas.org
KEAP1 (Kelch-like ECH-associated protein), a cytoplasmic repressor of the oxidative stress responsive transcription factor Nuclear factor erythroid...
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A lot of great talks this year at the @A_P_S_A @JointMeeting ! Looking forward to next year already. @uchicagomstp
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