Thanks to @ginny_farias and @JKlumperman for the supervision during this project. Also major thanks to the co-authors @Anna_E_George , Tineke Veenendaal, Suzanne van Dijk, Frederik Verweij for the collaborative effort!

🧵These APP-CTFs were subsequently secreted via small vesicles called exosomes as a form of waste management - a process that could potentially contribute to disease propagation in Alzheimer's Disease

🧵HOPS dysfunction resulted in a drastic accumulation of APP in static and catalytically inactive endosomes that contained BACE1, but lacked the γ-secretase subunit PSEN2. Consequently, this led to the intracellular build-up of APP-CTFs

🧵In this study, we show that the HOPS complex, a regulator of endo-lysosomal fusion, is surprisingly essential for retromer-mediated recycling of APP from endosomes to the TGN

🧵APP is involved in various neuronal functions, but it is also the source of toxic Aβ and APP-CTFs in Alzheimer's Disease patients. APP trafficking and processing highly depend on endo-lysosomal functioning, however effectors that govern these processes are not fully understood

I am happy to share our work on the role of the HOPS complex in APP trafficking and processing! 💫 Out now on bioRxiv: https://t.co/AAwLzQEEEo

Cool work from my colleague! Check it out!

The latest preprint from our lab. Congratulations to the first author @EdenJess and thank you to all our wonderful collaborators. @TheCIMR @CellBiol_MRCLMB @JLS_Lab

Yesss!! It's out in @Dev_Cell!🥳Check it out to see lots of new exciting data on the axonal ER and local translation compared to the pre-print. Thanks to the reviewers for their insights that helped improve this study. And many thanks to all involved, a real team effort!

Fun times during the Farías lab outing @ginny_farias! For those wondering: this is not our lab merchandise 🙃

It's out!😭🕺First preprint as a PhD at the Farias Lab @ginny_farias where we investigated biosynthetic lysosomal membrane protein trafficking, delivery and interactome with Protein Origin, Trafficking And Targeting to Organelle Mapping (POTATOMap)🥔 https://t.co/vmiYfjIx7F

Thank you Claudia for the nice discussions and insightful presentation! It was a pleasure to have you here!

It was a pleasure to be interviewed by Amelia Glazier for JCS. It was fun! Cell scientist to watch – Ginny G. Farías

Very happy to share our perspective article on polarized protein secretion in neurons! We explore each step in the sorting process, from ER to PM, highlighting the latest insights. @ginny_farias https://t.co/WPJeADx82y

📢Up for an exciting PhD journey at the crossroads of high-resolution electron microscopy🔬and cancer biology🧬? We have an open #PhD position on "Nanoscale imaging of cancer organoids" 🔬🥼🧪🧫🧬 https://t.co/AuFUNapDqO #phdpositions

Spatial snapshots of amyloid precursor protein intramembrane processing via early endosome proteomics https://t.co/cxpWmLzF1w

Farias Lab - PhD students in action - NWO biophysics. Nice meeting! #proudPI @nazoozkan, @DerkDraper, @MaiDeeNguyen, @HaNguyen1107

Recruitment of clathrin to intracellular membranes is sufficient for vesicle formation

Want to make some ORANGE CAKE? My first publication with @hdmacgillavry, Arthur de Jong and @Jelmerwillems is fully online! Duplex Labeling and Manipulation of Neuronal Proteins Using Sequential CRISPR/Cas9 Gene Editing https://t.co/F1lqtkRz1F. Here are the key messages🧵

Early Endosome Capture Proteomics and its Application to Amyloid Precursor Protein Intramembrane Processing by β and γ-Secretases https://t.co/yK5WCxIdUE #biorxiv_cellbio