Dr. Anneliese 💖💜💙
@AMMGest
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Postdoc with @jinzhanglab. PhD, UC Berkeley from @millerchembio. I like microscopy and fluorescent sensors and also TTRPGs. She/her.
Joined June 2020
Given the state of the world and this website, we’re moving our socials primarily to Instagram and Bluesky, where we just made an account! Find us at https://t.co/qpIPRJiYf7 🦋 We’ll have a small presence here for a little bit, but we’ll be winding it down.
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Also, I’m still here intermittently, but I’m also trying out the other place! Same handle. 🦋
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Looking for a fluorescent biosensor and don’t know where to start? Want a comprehensive overview of the field? Check out our new review in @ACSChemRev online now as an ASAP article! From co-first authors @AMMGest @AyseZSahan @yanghao_zhong
https://t.co/YId0ttkqkf
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Thanks to everyone who came to our table at The Nat’s birthday event this past Saturday! We had a great time chatting with the community, making hydrogels, and extracting DNA from strawberries!
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Protein Kinase C (PKC) enzymes are critical signaling molecules, but their regulation at emerging signaling hubs remains unclear. In a study led by postdoc Qi Su @chrisqs5 , we developed a sensitive fluorescent biosensor of PKC, ExRai-CKAR2! https://t.co/71xbUggaxi
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QSS will be at The Nat’s Big Birthday Block Party on Saturday, October 19th! Join us there — and if you want, come volunteer for a little while! Talk about your science and help run science activities! RSVP here to volunteer: https://t.co/BgwAcqv3F6
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Thank you everyone for coming to our fall kickoff event! We had some great snacks and conversation! Stay tuned for news on our next event, and hope to see y’all there!
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🧪A team of researchers, led by @HelenFarrants & @ERSchreiter, developed a new way for engineered protein biosensors & bright, fluorescent Janelia Fluor dyes to work together, creating a new biosensor for measuring physiological signals in live animals.➡️ https://t.co/oVKLIGmsRP
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In one week we’ll be having a social get together and kickoff to the academic year! Come join us on September 27th from 4-7 pm for snacks, drinks, and chats! RSVP here:
linktr.ee
Queer scholar community, open to all Queer and ally scientists of San Diego area
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So excited to be running a hands-on microscopy workshop for @bummp_ucsd September 16-18 (fun fact - one of these days is my 45th birthday!). Will be running with @UCSD_NIC and the inestimable @Neal_Lab. BUMMP students - send an email if you’re interested! Sponsors welcome :-)
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Please share! Here's our latest preprint on the university-wide mentorship program @bummp_ucsd, highlighting its profound impact on biology students from all backgrounds. In 4 years, this pioneering initiative has served over 1,350 mentees!!! https://t.co/lC040QeITh
biorxiv.org
In the face of a challenging climate that is resistant to Diversity, Equity, and Inclusion (DEI) efforts, there is a critical need for a support structure for retaining students, particularly those...
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Check out the brief "tweetorial" @JLazzariDean made when it went up on biorxiv for more info!
A Jack of All Trades, A Real "Renaissance Dye"! Announcing CRhOMe ("chrome"), a new voltage sensitive dye that we developed at @MillerChemBio CRhOMe is great for both monitoring action potentials and quantifying voltage. Preprint: https://t.co/gOR1e5HzyS
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Happy #FluorescenceFriday everyone! Our "Renaissance Dye" CRhOMe (and my co-first author paper from my PhD) is now published and online at PNAS: https://t.co/dkb1q0rjeY Really proud of this work and everyone from the @MillerChemBio lab who contributed to this project.
pnas.org
Biological membrane potentials, or voltages, are a central facet of cellular life. Optical methods to visualize cellular membrane voltages with flu...
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Check out our new article in @TrendsChemistry! We review how 3-functionality can be leveraged to drastically alter the properties of xanthene fluorophores and unlock new applications in biological imaging. #FluorescenceFriday
https://t.co/V5I1wKBjBH
cell.com
Fluorescent dyes are an indispensable part of the scientific enterprise. Xanthene-based fluorophores, such as fluorescein and rhodamine, have been in continual use across numerous fields since their...
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@vincentngrenier And of course thank you to @BerkeleyMIC for access to the lifetime scope that made this work possible. Couldn't do it without the excellent imaging core facility with its instrumentation and expertise!
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We characterized two genetic targeting systems with modified VoltageFluor dyes (synthesized by @vincentngrenier) that bind to either SpyTag or HaloTag. VoltageSpy is a little more sensitive, while VF-HaloTag gives better staining, and we demonstrate it in cultured neurons.
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Earlier in my time in the Miller lab we showed that you can estimate resting membrane potential with VoltageFluor dyes and FLIM. https://t.co/75EpJ2yaDT But indiscriminate staining of all membranes makes looking at complicated cultures difficult – enter targeted voltage dyes.
elifesciences.org
Fluorescence lifetime imaging microscopy, paired with fluorescent, voltage-sensitive dyes, provides a method for measuring and quantifying membrane potentials of living cells.
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Happy #FluorescenceFriday! Out now is the latest work from my PhD with @millerchembio showcasing two chemical-genetic targeting systems for VoltageFluor dyes, enabling cell-type specific monitoring of membrane potential – read out optically with FLIM.
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We are hiring! If you are excited about building microscopes, voltage imaging, or techniques that can improve biological measurements I'd love to hear from you. Postdoc ad linked below, and we will be hiring at all levels soon. https://t.co/Jc7DtxHfiw
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Awesome summary of places you can share published imaging datasets
This is a valid concern that comes up frequently. Two things: 1. People will look at imaging data associated with papers. We certainly require and look for such datain our lab. 2. There are solutions. See this article by Kayla Jackson:
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